In Tinker Bell and the Lost Treasure, Fawn trains tiny pink butterflies to move from a brush after she signals it.
In Tinker Bell and the Great Fairy Rescue (film), it shows that Butterflies have symmetrical wings that are painted by the Animal-talent fairies, however mistakes can be made and the butterfly might come out with unsymmetrical painted wings. Butterflies are also shown as a subject to Dr. Martin Griffiths scientific research. He captures the butterflies, write and take notes on them. A butterfly with mismatched patterns was seen by Lizzy Griffiths and captured by her father. In the end the butterfly is freed by Tinker Bell.
In The Realm of the Never Fairies, it explains that butterflies fly wherever they want, not in straight line and do not pay attention to cheers and encouragement, being intelligent and "extremely mischievous" by nature, yet "obnoxious creatures." The only ones with enough patience to work with them are the few Butterfly-herding-talent fairies that takes the herd of butterflies, over fifty in number, to pasture in a meadow beside Havendish Stream, and their eggs becomes caterpillars (in which woolly caterpillars are raised for their fur). In Butterfly races fairies use "clever tactics" such as casting shadows of large birds to chase the butterflies towards the goal.
In Tinker Bell's Adventure, two butterflies were given as cards and the game cursor was a pink butterfly.